فهرست نشریات علمی دسترسی آزاد ایران

Humans can be exposed to the pesticide chlorpyrifos from food residues. Exposure in low doses and long periods causes disturbances in the balance of the intestinal microbiota, thereby disrupting the production of gut metabolites, namely short-chain fatty acids. SCFAs have a role in insulin sensitivity. This study aimed to determine the effect of chlorpyrifos exposure on SCFA and its impact on the onset of insulin resistance. Thirty Wistar rats were placed into five groups based on the length of the exposure: without exposure (control), 7 days (P1), 14 days (P2), 28 days (P3), and 56 days (P4). Chlorpyrifos at 5 mg/kg body weight was orally given once a day. SCFA was analysed using GCMS, and the HOMA-IR score was used to measure Insulin resistance. For 56 days, oral administration to chlorpyrifos reduced total SCFA levels while increasing fasting blood glucose, insulin, and HOMA-IR values compared to the control group. Sub-chronic exposure to chlorpyrifos reduced SCFA levels and increased HOMA-IR levels in Wistar rats. It can be suggested that the time needed to make an animal model of insulin resistance due to chlorpyrifos exposure requires 45 days or more.