MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma
نویسندگان
1 Jiangsu Medical Engineering Research Center of Gene Detection, Xuzhou Medical University, Xuzhou, Jiangsu, China
2 Jiangsu Medical Engineering Research Center of Gene Detection, Xuzhou Medical University, Xuzhou, Jiangsu, China
3 Department of Forensic Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, China
4 Jiangsu Medical Engineering Research Center of Gene Detection, Xuzhou Medical University, Xuzhou, Jiangsu, China
5 Department of Forensic Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, China
6 Jiangsu Medical Engineering Research Center of Gene Detection, Xuzhou Medical University, Xuzhou, Jiangsu, China
7 Department of Human Anatomy, Xuzhou Medical University, Xuzhou, Jiangsu, China
8 Department of Forensic Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, China
9 Department of Forensic Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, China
10 Jiangsu Medical Engineering Research Center of Gene Detection, Xuzhou Medical University, Xuzhou, Jiangsu, China
11 Department of Basic Pathology, Pathology College, Qiqihar Medical University, Qiqihar, Heilongjiang, China
12 NGS center, Hangzhou D.A. Medical Laboratory Co., Ltd., Hangzhou, Zhejiang, China
doi
10.22038/ijbms.2022.65886.14492چکیده
Objective(s): Esophageal cancer is one of the most common cancers with high incidence and mortality rates, especially in China. MicroRNA (miRNA) can be used as a prognostic marker for various human cancers. This study aims to detect suitable miRNA markers for esophageal squamous cell carcinoma (ESCC). Materials and Methods: Our previous gene expression data of ESCC cells and the data from GSE43732 and GSE112840 were analyzed. The expression of miR-574-5p in ESCC patients and controls was analyzed by real-time quantitative PCR. The effect of miR-574-5p on proliferation was detected by real-time cell analysis (RTCA) and EdU proliferation assay after cell transfections. The target gene small C-terminal domain phosphatase 1 (CTDSP1) of miR-574-5p was validated by luciferase reporter assay and western blotting.Results: In the current study, the bioinformatics analysis found miR-574-5p up-regulated in ESCC. The qPCR assay of 26 ESCC and 13 adjacent/ normal tissues confirmed these results. We further demonstrated that miR-574-5p overexpression promoted cell proliferation. Then the dual-luciferase reporter assay and the rescue experiment suggested that CTDSP1 was a direct target of miR-574-5p.Conclusion: MiR-574-5p played an oncological role in ESCC by interacting and negatively regulating CTDSP1. These results provided a deeper understanding of the effect of miR-574-5p on ESCC.
