Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway
نویسندگان
1 Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
2 Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
3 Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
4 Zhejiang University Mingzhou Hospital, Ningbo, 315000, China
5 Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
6 Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
7 Zhejiang University Mingzhou Hospital, Ningbo, 315000, China
8 Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
9 Zhejiang University Mingzhou Hospital, Ningbo, 315000, China
10 Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
11 Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
12 Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China
doi
10.22038/ijbms.2021.56781.12678چکیده
Objective(s): This research was designed to determine the role of irisin in lipopolysaccharide (LPS)-induced endometritis in female mice. Materials and Methods: Animals were randomly assigned into sham, sham + irisin, LPS, LPS + irisin (0.1, 1, 10 μg/kg), and LPS + irisin + compound C groups. Histological features and expression of AMPK, NF-κB, inflammatory mediators, and oxidative stress markers were compared among different groups. Results: The results showed that LPS resulted in obvious uterus damage, meanwhile, the inflammatory mediators (COX-2, iNOS, IL-1β, IL-6, and TNF-α), as well as NF-κB in the uterine tissue, were significantly increased and the level of adenosine monophosphate-activated protein kinase (AMPK) was reduced. Nevertheless, pretreatment with irisin reversed the phenomena caused by LPS. Interestingly, compound C (AMPK inhibitor) abolished irisin’s effects on the uterus, which suggested that irisin’s beneficial function was achieved through regulating the AMPK-NF-κB pathway. Moreover, LPS-induced alterations of oxidative factors (MnSOD, GSH, and MDA) were reversed significantly by pretreatment with irisin. This data indicated irisin’s beneficial function was also related to antioxidation besides anti-inflammation. Conclusion: Our study implies that irisin is a potential therapeutic agent for endometritis.
