A Preliminary Protocol for Induction of Tuberculosis Spondylitis by Mycobacterium Tuberculosis Strain H37R: In-vivo New Zealand White Rrabbits Model
نویسندگان
1 Department of Pharmacology and Therapeutics School of Medicine, Universitas Sumatera Utara, Indonesia
2 Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
3 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
4 Stem Cell and Tissue Engineering Cluster, IMERI Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia, Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo General Hospital, Jakarta 10430, Indonesia
5 Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Sumatera Utara-Adam Malik General Hospital, Medan, Indonesia
6 Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia
7 Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Sumatera Utara-Adam Malik General Hospital, Medan, Indonesia
8 Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Sumatera Utara-Adam Malik General Hospital, Medan, Indonesia
9 Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Sumatera Utara-Adam Malik General Hospital, Medan, Indonesia
10 Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Sumatera Utara-Adam Malik General Hospital, Medan, Indonesia
doi
10.26655/JMCHEMSCI.2022.7.19چکیده
Background: Proper animal model is a critical prerequisite of any experimental study, while it is a lack for tuberculosis spondylitis. This study aimed to validate a protocol for induction of tuberculosis spondylitis in an animal model.Methods: Ten New Zealand white rabbits were used and divided randomly into four experimental groups (n = 8), with 2 rabbits in each group, and the control group (n = 2). A 0.2 mL suspension of 108 CFU/mL H37Rv strain M. tuberculosis was delivered into the drilled hole with a depth of 6-10 mm at the midpoint of the 12th thoracal corpus compared with the saline in the control group. Evaluation of imaging examination by using plain X-ray was done within the targeted time followed by euthanasia protocol.Results: A survival rate of 100% was observed in the present study with all rabbits having stable vital signs during the incubation period. Group 3 with an incubation period of 6 weeks showed the highest success rate for inoculation of M. tuberculosis bacteria in which 7 samples were positive. This was followed by group 2 (75%, incubation 4 weeks), group 1 (37.5%, incubation 2 weeks), and group 4 (12.5%, incubation 8 weeks). The high positive rates were also reported by using culture and PCR staining, 62.5% and 75%, respectively. A slight destruction of the vertebral body was observed in both groups 2 and 3 starting at 4 weeks, postoperatively. Histopathology specimen exhibited an infiltration of numerous inflammatory cells. Conclusions: Establishment of spondylitis tuberculosis model by using New Zealand white rabbit could be successfully formed through 0.2 mL suspension of 108 CFU/mL M. tuberculosis strain H37Rv with a direct inoculation method towards the midpoint of the 12th thoracal vertebral body. In addition, this model showed a high positive rate with a 100% survival rate in both control and 4 treatment groups.
