Evaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method
نویسندگان
1 Jinhua Polytechnic, Jinhua 321007, Zhejiang, China
2 Jinhua Central Hospital, Jinhua 321000, Zhejiang, China
3 Jinhua Polytechnic, Jinhua 321007, Zhejiang, China
4 Jinhua Polytechnic, Jinhua 321007, Zhejiang, China
5 The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China
6 Jinhua Polytechnic, Jinhua 321007, Zhejiang, China
7 Jinhua Polytechnic, Jinhua 321007, Zhejiang, China
8 Jinhua Polytechnic, Jinhua 321007, Zhejiang, China
9 Jinhua Polytechnic, Jinhua 321007, Zhejiang, China
doi
10.22038/ijbms.2019.31611.7611چکیده
Objective(s): In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo. Materials and Methods: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral dose of a cocktail solution containing phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam. Then, we obtained blood in specific durations for the determination of plasma concentration of the probe drugs using UPLC-MS/MS. We also evaluated the pharmacokinetic parameters using the DAS 2.0 software. Results: We found that various concentrations of lentinan increased the activity of rat CYP1A2, CYP3A4, CYP2D6, and CYP2C19 but not CYP2C9. Conclusion: These findings suggest that clinical application of lentinan combination with CYP3A4, CYP1A2, CYP2C19, or CYP2D6 should be given careful consideration as this may lead to herb-drug interactions and hence treatment failure.
