Staging of colorectal cancer using serum metabolomics with 1HNMR Spectroscopy
نویسندگان
1 Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran
2 Amir Alam Hospital, Amir Alam, Tehran, Iran
3 Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran
4 Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran
5 Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. Iran
6 Amir Alam Hospital, Amir Alam, Tehran, Iran
7 Payam Noor University, Tehran, Iran
8 Payam Noor University, Tehran, Iran
9 Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran
10 Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. Iran
doi
10.22038/ijbms.2017.9022چکیده
Objective(s): Determination of stages of colon cancer is done by biopsy usually after surgery. Metabolomics is the study of all the metabolites using LC-MS and 1HNMR spectroscopy with chemometric techniques. The stages of colon cancer were detected from patients' sera using 1HNMR. Materials and Methods: Five ml blood was collected from 16 confirmed patients referred for colonoscopy. One group of eight patients were diagnosed with stage 0 to I colon cancer and the second group of 8 patients with II-IV stage colon cancer. Sera were sent for 1HNMR. The differentiating metabolites were identified using HMDB and the metabolic cycles from Metaboanalyst. Results: Six metabolites of which pyridoxine levels lowered, and glycine, cholesterol, taurocholic acid, cholesteryl ester and deoxyinosine increased. Conclusion: The different stages of cancer were identified by the main metabolic cycles such as primary bile acid biosynthesis, purine and vitamin B metabolic pathways and the glutathione cycle.
