Mild hypothermia reduces expression of Fas/FasL and MMP-3 after cerebral ischemia-reperfusion in rats
نویسندگان
1 Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
2 Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
3 Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
4 Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
5 Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
6 PuRen Hospital of WuHan, Wu han, China
doi
10.22038/ijbms.2014.2931چکیده
Objective(s):To investigate the effects of local mild hypothermia on the expression of Fas, FasL and MMP-3 after cerebral ischemia-reperfusion in rats. Materials and Methods:Male Wistar rats were divided into sham-operated group (Sham), normothermia group (NT), and hypothermia group (HT). MCAO/R model was established by Longa’s method, and reperfusion was allowed after 2 hr occlusion. Mild hypothermia (33±0.5℃) for 6 hr was initiated at the start of reperfusion. Immunohistochemistry was performed to determine expression Fas, FasL, and MMP-3. Results: Infarct volume was reduced in the hypothermia group (18.43±4.23%) compared with the normothermia group (24.76±5.76%) (P<0.05). In mild hypothermia group, numbers of Fas-positive and MMP-3 positive cells were significantly less than those of normothermia group (P<0.05). Neurological functional scores of mild hypothermia were significantly improved (P<0.05). Conclusion: Mild hypothermia decreases infarct volume after cerebral ischemia-reperfusion, reduces Fas and MMP-3 expression, but increases FasL in cerebral ischemia-reperfusion rats.
