Study the Antinociceptive Effect of Intracerebroventricular Injection of Aqueous Extract of Origanum Vulgare Leaves in Rat: Possible Involvement of Opioid System
نویسندگان
1 Neuroscience Research Center and Department of Physiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
2 Neuroscience Research Center and Department of Physiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
3 Neuroscience Research Center and Department of Physiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
4 Faculty of Medicine, Ardabil Azad University of Medical Sciences, Ardabil, Iran
5 Neuroscience Research Center and Department of Physiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran 2 Department of Physiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6 Departments of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
7 Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
doi
10.22038/ijbms.2013.1874چکیده
Objective(s): The aim of study was to investigate the antinociceptive effect of intracerebroventricular (ICV) microinjection of Origanum vulgare (ORG) extract and possible involvement of opioid receptors. Materials and Methods: Cannula was inserted into left ventricle of male rats. Five days after surgery Tail Flick Latency (TFL) was measured after ICV microinjection of, ORG (1, 3 and 6 μg / rat). Effective dose of ORG was injected ICV in concomitant with morphine (2 mg/kg, IP), naloxone (2 mg / kg, IP) and saline (0.5 μl/rat) and TFL was recorded. Results: The co- administration of ORG extract with morphine showed a significant increase in TFL and naloxone, pretreatment significantly inhibited the antinociceptive activity of ORG and morphine. Conclusion: The aqueous extract of ORG possesses antinociceptive activities in the tail-flick test in a dose dependent manner. ORG - induced antinociception may have been mediated by opioid systems.
