فهرست نشریات علمی دسترسی آزاد ایران

Silver nanoparticles were synthesized, followed by 50 mL of Ficus hispida ethyl acetate extract was mixed with 100 mL of 4 mM silver nitrate solution, and stirring at room temperature, with a color change from yellow to brown indicating silver ion reduction. Characterization included UV‒Vis spectroscopy for surface plasmon resonance, FT-IR for functional groups, XRD for crystallinity, FE-SEM and HR-TEM for morphology, and zeta potential and DLS for particle size and stability. The anticancer activity against MCF-7 cells was assessed via the MTT assay, and ROS were detected via fluorescence microscopy. The synthesized FH-Ag-NPs presented a distinct UV‒Vis absorption peak at 191.69 nm, confirming nanoparticle formation. FT-IR analysis revealed characteristic peaks at 3,423 cm⁻¹ (O-H stretching), 2,924 cm⁻¹ (C-H stretching), and 1,636 cm⁻¹ (C=O stretching), indicating the involvement of biomolecules in nanoparticle stabilization. The zeta potential value of -31.0 mV suggested good colloidal stability. The DLS results revealed an average particle size of 122.3 nm with a polydispersity index of 0.368, reflecting moderate size uniformity. The XRD patterns confirmed the crystalline nature, with peaks at 10.67°, 27.65°, and 28.18°. Electron microscopy revealed that the spherical nanoparticles were 64–100 nm in size with minimal aggregation. EDAX confirmed that elemental silver was the main component. The FH-Ag-NPs exhibited dose-dependent cytotoxicity to MCF-7 cells, reducing viability from 85.73% (5 µg/mL) to 42.95% (25 µg/mL). Fluorescence microscopy revealed increased reactive oxygen species generation at higher concentrations, indicating oxidative stress-induced cytotoxicity. These results confirm the successful green synthesis and the FH-Ag-NPs promising anticancer potential.