Green Extraction, Phytochemical Profiling, and Biological Evaluation of Allium Humile Hydroalcoholic Extract
نویسندگان
1 School of Pharmacy, Sri Balaji Vidyapeeth (Deemed to be University), Pondicherry- 607402, India
2 Department of Pharmaceutics, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, India
3 Department of Pharmacognosy, Goenka College of Pharmacy, Lachhmangarh, Sikar, Rajasthan 332315 Ruhs, Jaipur, India
4 Department of Pharmaceutical Chemistry, Arulmigu Kalasalingam College of Pharmacy, Krishnan Koil, Tamil Nadu, India
5 Department of Pharmacology, GITAM School of Pharmacy, GITAM (Deemed to be University), campus Hyderabad, Telangana-502329, India
6 Department of Pharmacy Practice, Teerthanker Mahaveer College of Pharmacy, Teerthanker Mahaveer University, Moradabad 244001, India
7 Department of Pharmaceutical Chemistry, Telangana Social Welfare Residential Pharmacy College for Women Beside the Model School, Anantharam Road, Mahbubabad, Telangana, India
8 Department of Pharmacology, Vels Institute of Science, Technology & Advanced Studies (VISTAS), Velan Nagar, P.V. Vaithiyalingam Road, Pallavaram, Chennai – 600 117, Tamil Nadu, India
doi
10.48309/AJGC.2026.540330.1807چکیده
This study explored the anti-inflammatory and analgesic potential of Allium humile (AH) extract obtained through a green chemistry approach. The hydroalcoholic extract was prepared via cold maceration using ethanol:water (70:30 v/v), yielding 35.73 g from 300 g of powdered plant material, with an extraction efficiency of 11.91 %. Physicochemical analysis revealed standard values for total ash (6.83%), acid-insoluble ash (1.72%), water-soluble ash (3.90%), alcohol-soluble extractives (15.2%), water-soluble extractives (12.5%), and pH (6.3), indicating a good extract quality. Phytochemical analysis confirmed the presence of flavonoids, alkaloids, phenolics, tannins, glycosides, saponins, and terpenoids. The anti-inflammatory efficacy was investigated using carrageenan-induced paw edema, adjuvant-induced arthritis, and acetic acid-induced vascular permeability models in rats, while analgesic activity was evaluated using tail immersion and von Frey filament assays. Administration of AH extract at doses of 200 and 400 mg/kg showed significant anti-inflammatory and analgesic effects comparable to those of diclofenac sodium. These results indicate that AH is a viable source of bioactive substances for the management of inflammation and pain via natural therapeutic approaches.
