فهرست نشریات علمی دسترسی آزاد ایران

Crossandra infundibuliformis, a traditionally used medicinal plant, was evaluated for its phytochemical composition, antioxidant capacity, and hypoglycemic potential using bioactivity-guided fractionation and experimental models. Different preparations of the stem of C. infundibuliformis were subjected to phytochemical screening, fluorescence analysis, and quantitative assessment of the total flavonoid and phenolic content. Antioxidant potential was determined through inhibition assays of DPPH, α-amylase, and α-glucosidase, while acute oral toxicity was evaluated according to OECD guidelines. This study examined several parameters in diabetic Wistar rats that had been induced with streptozotocin (STZ), including the oral glucose tolerance test (OGTT), body weight monitoring, blood glucose estimation, serum insulin levels, and antioxidant enzyme activity (SOD, CAT, GSH, and MDA). Among the tested extracts, the ethanolic extract demonstrated the highest phenolic (241.28 µg/mL) and flavonoid (333.38 µg/mL) content. The ethyl acetate fraction exhibited potent DPPH radical scavenging activity (99.19%) and α-amylase inhibition (59.35%), while the n-butanol fraction inhibited α-glucosidase (77.24%) compared to acarbose. In vivo studies revealed significant antihyperglycemic effects, with OGTT showing a reduction in glucose levels from 161.00 ± 4.36 mg/dL (15 min) to 93.67 ± 2.08 mg/dL (120 min). Long-term treatment reduced blood glucose from 241.5 ± 2.43 mg/dL (Day 7) to 126.3 ± 10.25 mg/dL (Day 28) and elevated serum insulin to 820 ± 96.22 pg/mL. Antioxidant enzyme levels improved significantly, with increased SOD, CAT, and GSH levels and decreased MDA levels. Overall, the findings confirm that Crossandra infundibuliformis possesses substantial antioxidant and hypoglycemic properties, supporting its traditional use and highlighting its potential as a candidate for antidiabetic phytomedicine development.