فهرست نشریات علمی دسترسی آزاد ایران

Due to their diverse medicinal potential, marine plants, especially red seaweeds, have drawn increasing attention in recent years. Therefore, red seaweed Amphiroa fragilissima was evaluated for its anti-diabetic and antioxidant properties through in vitro enzyme inhibition assays. The extraction process was performed with the help of ultrasonication which extracts maximum number of active metabolites. Extracts of A. fragilissima in petroleum ether, ethyl acetate, methanol, and hydroethanol were tested against α-amylase and α-glucosidase. The IC50 values for α-glucosidase inhibition were >320 μg/ml, 236 μg/ml, 141.75 μg/ml, 63.13 μg/ml for the extracts, and 25.96 μg/ml for voglibose. For α-amylase, the IC50 values were >320 μg/ml, 72.42 μg/ml, 18.41 μg/ml, 9.14 μg/ml for the extracts, and 3.12 μg/ml for acarbose. The hydroethanolic extract showed the strongest inhibition of both the enzymes. The petroleum ether extract exhibited no significant inhibition of either enzyme. Antioxidant activity was assessed using the DPPH, ABTS, phosphor-molybdenum, and hydrogen peroxide assays. For DPPH scavenging, IC50 values were >320 μg/ml, 187 μg/ml, 131.66 μg/ml, 63.97 μg/ml for the extracts, and 11.59 μg/ml for ascorbic acid. For ABTS scavenging, the IC50 values were >320 μg/ml, 111.41 μg/ml, 88.89 μg/ml, 44.06 μg/ml, and 16.85 μg/ml for ascorbic acid. The total antioxidant capacity was highest for the methanol and hydroethanolic extracts (95.72% and 95.06%, respectively). The H2O2 scavenging potential was the strongest for α-tocopherol, followed by the methanol and hydroethanolic extracts. Overall, ethyl acetate and methanol extracts demonstrated significant anti-diabetic and antioxidant activities (p≤0.05), whereas petroleum ether showed no significant effects. The results of this study suggest that Amphiroa fragilissima possesses significant anti-diabetic and antioxidant potential, particularly in its methanol and hydroethanolic extracts. These findings support the potential use of these extracts in the development of natural therapeutic agents to manage diabetes and oxidative stress-related conditions