Chemical Composition, Larvicidal and Ovicidal Activities of Majorana Hortensis Essential Oil Against Spodoptera Frugiperda (J.E. Smith) and Their Impact on Detoxification Enzymes
نویسندگان
1 Insect Population Toxicology Department, Central Agricultural Pesticides Laboratory, Agriculture Research Center, 12618, Giza, Egypt
doi
10.48309/pcbr.2026.533824.1443چکیده
Plant essential oils (EOs) are considered ideal alternatives to conventional insecticides for pest control. In this study, the chemical composition of Majorana hortensis Moench (Lamiaceae) was assessed by Gas Chromatography-Mass Spectrometry (GC-MS), and its larvicidal and ovicidal activity against fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera; Noctuidae) and their impact on detoxification enzymes were evaluated. GC–MS analysis revealed that the main constituents of M. hortensis EO were terpinen-4-ol (29.96%), γ-terpinene (11.34%), and trans-sabinene (10.81%). M. hortensis EO was the most toxic compound with a median lethal concentration (LC50) of 1.81, 0.68 and 0.24 mg/mL after 24, 48 and 72 h post-treatment, respectively. The results indicated that M. hortensis EO was most toxic to eggs. Furthermore, the development time of the larval, pupal and adult stages was significantly affected after M. hortensis EO exposure, compared to the control treatment. In S. frugiperda larvae, M. hortensis EO treatment at LC50 reduced the enzymatic activity of esterase (EST), glutathione S-transferase (GST), and acetylcholinesterase (AChE), with that AChE being more sensitive (92.67%), suggesting that AChE may be the main target. Overall, this study demonstrated that M. hortensis EO has the potential to be implemented as a novel eco-friendly insecticide against S. frugiper