فهرست نشریات علمی دسترسی آزاد ایران

In this work, the kinetic factors (affinity factor (Km) and maximal velocity (Vmax)) and the catalytic performances (operational performance and pH stability) of the peroxidase-like MnFe2O4 nanozymes were experimentally assessed. Initially, the MnFe2O4 nanozymes were synthesized and then characterized using SEM and VSM analysis, revealing spherical particles with a size of 118-305 nm and a magnetization saturation value of 21.2 emu g-1. Thereafter, the o-phenylenediamine (OPD)-mediated enzyme assay was utilized for determining the reaction rates and consequently constructing the saturation curves. The Michaelis–Menten kinetic model was used as the standard kinetic model of enzymatic reactions, while the linear plot of Lineweaver–Burk was constructed to provide precise values of parameters. The results indicated a Vmax of 0.12 µM s-1 for the MnFe2O4 nanozymes. Besides, the substrate affinity factor, Km, was estimated at about 27.7 mM for the MnFe2O4 nanozymes, exhibiting the high affinity of MnFe2O4 nanozymes toward OPD. The operational performance studies exhibited that the nanozymes retained about 85.3% after 10 cycles. Besides, the pH stability study showed an optimal pH of 7.0 for the MnFe2O4 nanozymes, as same as the native enzyme. The results of this research proved that MnFe2O4 nanozymes are high-throughput nanozymes with excellent catalytic efficiency and operational performance.