فهرست نشریات علمی دسترسی آزاد ایران

Background: Cutaneous wounds are among the most common disruptions to skin integrity. Traditional methods for improving wound healing have often proven ineffective, highlighting the need for novel and more effective approaches. Recently, biologically based therapies have gained attention due to their safety and biocompatibility. Stem cells, in particular, have been widely used to treat wounds and have shown promising results. However, it is believed that the beneficial effects of stem cells are primarily mediated by the extracellular vesicles they secrete, specifically exosomes. While the functions of exosomes derived from cells such as mesenchymal stem cells (MSCs) have been extensively studied, there is limited data on exosomes derived from fibroblasts, and their specific roles remain unclear. This study investigates the potential of fibroblast-derived exosomes (FB-EXOs) to promote wound healing in skin cells under in vitro conditions.Methods: Exosomes isolated from fibroblast using an isolation kit were first characterized by field emission scanning electron microscopy (FESEM), dynamic light scattering (DLS), and the Bradford assay. Subsequently, they were applied to skin cells in MTT, trypan blue, scratch, and angiogenesis (sprouting) assays to evaluate their healing effects.Results: In this study, the isolated exosomes were observed to have diameters less than 200 nm by DLS and exhibited a spiral shape under FESEM. Their protein concentration was measured at 214 μg/ mL. These particles increased cell viability by up to 38.38% in the MTT assay and by 15% in the trypan blue assay. They also increased the cell migration rate by about 35% and the wound area in the high-dose exosome treatment group was completely closed. Finally, exosome treatment significantly increased the tube formation activity of human umbilical vein endothelial cells (HUVECs) compared to both the control and VEGF treatment groups.Conclusion: The present study showed that FB-EXOs can facilitate wound healing by promoting cell proliferation, migration, and angiogenesis in skin tissue.