Nanocurcumin-Derived Conditioned Media from Dental Pulp Stem Cells: Synthesis, Characterization, and Antimicrobial Activity
نویسندگان
1 Department of Restorative Dentistry, School of Dentistry, Kermanshah University of Medical Science, Kermanshah, Iran
2 Department of Pediatric Dentistry, School of Dentistry, Kermanshah University of Medical Science, Kermanshah, Iran
3 Faculty of Health and Life Sciences, INTI International University, Nilai 71800, Malaysia
4 Division of Dental Biomaterials, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
5 Advanced Dental Sciences and Technology Research Center, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
6 Department of Prosthodontics, School of Dentistry, Kermanshah University of Medical Science, Kermanshah, Iran
7 Department of Periodontics, School of Dentistry, Kermanshah University of Medical Science, Kermanshah, Iran
doi
10.48309/jaoc.2026.559744.1361چکیده
The purpose of this research was to examine the potential antibacterial properties of a conditioned medium derived from nanocurcumin-treated dental pulp mesenchymal stem cells (DPSC). Scanning electron microscopy and energy-dispersive X-ray spectroscopy were used to study the physicochemical properties of curcumin nanoparticles. The MTT assay was used to determine the optimal dose of nanocurcumin for treating DPSC cells. Using ROS measurements, the research investigated nanocurcumin's antioxidant effects on DPSC cells. Separated into two groups, one received nanocurcumin treatment, and the other received no treatment at all (control). Next, it was examined whether or not the DPSC cell supernatant had any antimicrobial or antifungal characteristics. The physicochemical attributes of nanocurcumin, such as its purity, shape, and nanoparticle size, were determined to be suitable in this study. Furthermore, it enhances antioxidant capabilities at the IC50 concentration and is non-toxic at dosages ranging from 0 to 5 (μg/mL). In addition, it raises DPSCs. Based on the antibacterial results, CM-CUR-NP at different doses kills 95% of Candida albicans and decreases the viability of Staphylococcus mutans. According to the study's findings, CM-CUR-NP can be effectively used in dentistry to combat both bacteria and fungi, which could lead to better overall health for individuals.
