Development of Superparamagnetic Iron Oxide Nanoparticles for Advanced MRI Contrast Enhancement
نویسندگان
1 Faculty of Dentistry, Dagestan State Medical University, Makhachkala, Republic of Dagestan, Russia
2 Faculty of Medicine, Stavropol State Medical University, Stavropol, Russia
3 Department of Social Sciences and Humanities and Technologies, National Research Moscow State University of Civil Engineering, Moscow, Russia
4 Faculty of Medicine, Saratov State Medical University named after V.I. Razumovsky, Saratov, Russia
5 Faculty of Medicine, Saratov State Medical University named after V.I. Razumovsky, Saratov, Russia
6 Faculty of Medicine, Dagestan State Medical University, Makhachkala, Republic of Dagestan, Russia
7 Faculty of Medicine, Dagestan State Medical University, Makhachkala, Republic of Dagestan, Russia
8 Faculty of Medicine, Stavropol State Medical University, Stavropol, Russia
9 Department of International Relations and Foreign Policy of Russia, Institute of International Relations and Socio-Political Sciences, faculty of Socio-Political Science, Moscow state linguistic University, Moscow, Russia
10 Faculty of Pediatrics, Saratov State Medical University named after V.I. Razumovsky, Saratov, Russia
11 Faculty of Medicine, Astrakhan State Medical University, Astrakhan, Russia
12 Faculty of Pediatrics, Rostov State Medical University, Rostov-on-Don, Russia
doi
10.48309/jaoc.2025.522350.1294چکیده
The present study is devoted to the development of a new magnetic resonance contrast agent based on superparamagnetic magnetite nanoparticles. The aim of the study is to create a contrast agent with improved characteristics compared to existing analogues. The process of obtaining includes two main stages such as the formation of the embryo and the growth of particles by aggregation. The size of the nanoparticles was controlled to achieve superparamagnetism. The concentration of iron in the solution was determined by X-ray fluorescence analysis. The phase composition was confirmed by X-ray phase analysis. Transmission electron microscopy and proton relaxometry were employed to evaluate the obtained solutions. The study showed that the optimal concentration of superparamagnetic nanoparticles in an aqueous solution is 0.5 mg/mL. The relaxation abilities of the synthesized particles were determined: for superparamagnetic particles (5-8 nm) - t ≈: 12.1 l/(mmol·s), t ≈: 30.1 l/(mmol·s); for ferrimagnetic particles (30-50 nm) - t ≈: 3.2 l/(mmol·s), t ≈: 35 l/(mmol·s). The cytotoxicity of the obtained nanoparticles was evaluated using an MTT test on fibroblast cells. It was found that at concentrations lower than 0.5 mg/mL, the particles do not exhibit a toxic effect on normal fibroblast cells. The research results demonstrate the feasibility of creating a new contrast agent for MR tomography based on synthesized nanoparticles of complex iron oxide. The developed contrast agent outperforms existing analogues in its characteristics and shows promising results in terms of biocompatibility.
