The Effect of Curcumin on Angiogenic and Proliferative Factors inHuman Endometriotic Cells
نویسندگان
1 Department of Cell, Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, Iran
2 Department of Stem Cells and Developmental Biology, Cell Science Research Centre, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
3 Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
4 Department of Genetics, Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
5 Department of Stem Cells and Developmental Biology, Cell Science Research Centre, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
6 Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
7 Department of Cell, Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, Iran
doi
10.22074/ijfs.2025.2029019.1684چکیده
Background: Endometriosis is a benign gynaecological disorder characterised by the presence of endometrial-liketissue outside the uterus. Proliferation of endometrial tissue and neoangiogenesis are essential factors in the developmentof endometriosis. Vascular endothelial growth factor (VEGF) and insulin‐like growth factors (IGF1, IGF2) canbe effective in neoangiogenesis and cell proliferation. Imprinted long non-coding RNA (lncRNA) H19 is involvedin endometriosis pathogenesis through the regulation of cellular proliferation and differentiation. Curcumin has antiangiogenic,anti-proliferative, and anti-invasive properties for various diseases, and it is hypothesised that it may havetherapeutic effects on endometriosis. This study aimed to evaluate the effects of curcumin on the expression of VEGF,IGF1, IGF2, and H19 lncRNA, as well as on cell migration and proliferation in endometrial stromal cells isolatedfrom women with endometriosis.Materials and Methods: In this experimental study, human normal endometrial stromal cells (N-ESCs) from healthyendometrial tissue and human eutopic endometrial stromal cells (EU-ESCs) from endometriosis patients were culturedand treated with different concentrations of curcumin (0-100 mM) for 72 hours. Then, the effects of curcuminon cell viability, proliferation, and migration were assessed using methylthiazole tetrazolium (MTT) and the scratchtest, respectively. Expressions of genes involved in angiogenesis (VEGF), proliferation (IGF1, IGF2), and the H19lncRNA gene were evaluated using real-time polymerase chain reaction (PCR).Results: Curcumin decreased cell migration and proliferation of endometriotic stromal cells in a time- and concentration-dependent manner. It also reduced VEGF expression and increased the expressions of H19, IGF1, and IGF2.Conclusion: The anti-migration effect of curcumin on EU-ESCs and its impact on the expression of proliferative andangiogenic genes indicate that curcumin can be a promising treatment for endometriosis.
