Fabrication and Characterization of Xylomannan/Fe3O4 Nanocomposite to Improve Post-Vitrification Viability of Mouse Immature Oocytes
نویسندگان
1 Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
2 Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
3 Department of Developmental Biology, School of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran
4 Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
doi
10.22074/ijfs.2025.2046101.1785چکیده
Background: Nano-biotechnology is a rapidly evolving field merging biology, engineering, and chemistry. Nanoparticles (NPs) with low toxicity and diverse properties, hold great promise for cryopreservation. Their ability to modify membrane permeability, optimize cryoprotectant entry and removal, tolerate freezing temperatures, enhance warming efficiency, and improve the heat transfer and thermodynamic properties of cryo-solution, makes them a game-changer for low-temperature preservation. This study investigated the potential of Fe3O4/Xylomannan nanocomposites (FXN) for improving mouse germinal vesicle vitrification. Materials and Methods: This study is an experimental research. Xylomannan, a polysaccharide derived from Enoki mushroom, exhibits cryoprotective properties. The nanocomposite was synthesized by coating Fe3O4 NPs with Xylomannan. Characterization techniques, including X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), and scanning electron microscopy (SEM) confirmed the successful synthesis and characterization of the Fe3O4/Xylomannan nanocomposite. Immature oocytes, GV DO (denuded oocytes) were obtained from 40 adult Naval Medical Research Institute (NMRI) mice and divided into 5 research groups (n-Vit, Vit, Vit+Fe3O4, Vit+Xylo, and Vit+FXN), therefore each experiment was repeated 5 times.Results: The cytotoxicity of different concentrations (0.002, 0.004, 0.008 and 0.016%) of Xylomannan, Fe3O4, and FXN was assessed on mouse embryo fibroblasts (MEF) at three time points (1, 2 and 7 days), using the MTT assay. While, the concentration 0.004% was the optimal dose of FXN, and selected for the subsequent experiments, 0.008 and 0.016% concentrations showed significant reduction in cell viability (P<0.05). Overall, the present study demonstrated that the Fe3O4/Xylomannan nanocomposite significantly improves post-vitrification survival rate in immature oocytes (****; P<0.0001) compared to the other vitrification groups, with no significant difference compared to the control group. Conclusion: The findings of this study reveal the potential of Fe3O4/Xylomannan nanocomposite as a promising cryoprotective agent for improving oocyte vitrification.
