Navigating The CeRNA Axis by Bioinformatics and Experimental Validation: Identification of ADIRF-AS1, miR-191-5p, and EGR1 as Key Players in Endometrial Carcinoma Progression
نویسندگان
1 Genetic Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
2 Biochemistry Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
3 Biochemistry Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
4 Biochemistry Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
5 Department of Gynaecology and Obstetrics, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
6 Biochemistry Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
7 Genetic Department of Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
doi
10.22074/ijfs.2024.2034827.1725چکیده
Background: Endometrial carcinoma (EC) is a significant gynecologic malignancy. Investigating competing endogenousRNA (ceRNA) networks, including long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messengerRNAs (mRNAs), offers insights into EC's molecular intricacies and may improve therapeutic and diagnostic strategies.This study focuses on the ceRNA axis, particularly the interactions of lncRNA-miRNA-mRNA-Gene, usingTCGA-UCEC database analysis and experimental validation.Materials and Methods: In this case-control study, differentially expressed lncRNAs (DElncRNAs), microRNAs (DEMIs),and genes (DEGs) were identified, highlighting ADIRF-AS1 as a potential target. Validated interactions betweenADIRF-AS1, miR-191-5p, and EGR1 were established, with significant ADIRF-AS1/EGR1 correlation. Protein-proteininteraction (PPI) analysis identified 21 proteins linked to EGR1, with gene ontology (GO) analysis revealing roles inmyeloid cell differentiation. Expression levels of genes, lncRNA, and microRNAs were evaluated by quantitative reversetranscription polymerase chain reaction (qRT-PCR). Western blot analysis was applied for protein evaluation.Results: RT-qPCR results showed that the RNA expression levels of ADIRF-AS1 and EGR1 genes in endometrialcancer and hyperplasia samples were significantly lower (P
