Laccase-Mimetic Activity of a Supramolecular Copper-β-Cyclodextrin Nanozyme for Colorimetric Dopamine Biosensing and Phenolic Pollutant Degradation
نویسندگان
1 Department of Inorganic chemistry, Faculty of Chemistry, University of Guilan, Rasht, Iran
2 Department of Inorganic chemistry, Faculty of Chemistry, University of Guilan, Rasht, Iran
3 Department of Inorganic chemistry, Faculty of Chemistry, University of Guilan, Rasht, Iran
doi
10.22036/abcr.2026.553340.2455چکیده
The accurate monitoring of dopamine, as a critical neurotransmitter in neurological function and disease progression, requires biosensors with high sensitivity and operational stability. In this study, we used a supermolecular copper-beta-cyclodextrin (Cu2-β-CD) complex as a high-performance laccase-mimetic nanozyme for the detection of dopamine. Kinetic studies based on the Michaelis-Menten model confirmed that Cu2-β-CD nanozymes with a higher Vmax performed better than the natural laccases, demonstrating superior catalytic efficiency. In addition, the synthetic nanozyme showed high stability under extreme pH conditions and maintained its long-term performance at ambient temperature, which eliminated the critical limitation of natural enzymes. Furthermore, the Cu2-β-CD nanozyme displayed oxidative activity towards phenolic pollutants, including 2,4-dichlorophenol, hydroquinone, catechol, and phenol, suggesting dual functionality in environmental remediation. Leveraging its high catalytic activity, we developed a colorimetric biosensor for dopamine detection in the linear range of 0.13-0.78 mM and a detection limit of 0.0026 mM (S/N = 3), combining biomimetic catalysis with simple optical readout. This work not only presents a robust alternative to natural laccase but also expands the potential of supramolecular nanozymes in biomedical sensing and pollutant degradation.
