Enhanced Spectrophotometric Detection of Phenylalanine Using a PCN-222(Zr)@Au NP Nanocomposite via Solid-phase Extraction
نویسندگان
1 Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 Institute of Mining, Oil and Energy, Mahs. C., Islamic Azad University, Mahshahr, Iran.
3 Institute of Mining, Oil and Energy, Mahs. C., Islamic Azad University, Mahshahr, Iran
4 Institute of Mining, Oil and Energy, Mahs. C., Islamic Azad University, Mahshahr, Iran
5 Institute of Mining, Oil and Energy, Mahs. C., Islamic Azad University, Mahshahr, Iran.
doi
10.22036/abcr.2025.538670.2398چکیده
In this study, a novel hybrid nanocomposite was synthesized by integrating gold nanoparticles into a zirconium-based metal-organic framework (PCN-222(Zr)). The resulting hybrid material was employed as an efficient adsorbent in a solid-phase extraction procedure for the preconcentration and spectrophotometric determination of phenylalanine. The synthesized nanocomposite was comprehensively characterized by Fourier Transform Infrared Spectroscopy, Powder X-ray Diffraction, Field-Emission Scanning Electron Microscopy, and Energy Dispersive X-ray Spectroscopy, confirming its successful fabrication and favorable physicochemical properties. Key experimental parameters such as pH, adsorbent dosage, solvent type, and extraction time were systematically optimized. Under optimized conditions, the method exhibited a wide linear range of 0.001-1.5 µmol L-1 with a detection limit of 3.6×10-5 µmol L-1. The developed platform displayed high selectivity toward phenylalanine with negligible interference from common coexisting species. Its applicability was validated through successful analysis of plasma samples, providing satisfactory recoveries and reproducibility. Compared with conventional spectrophotometric approaches, this nanocomposite-based platform offers superior sensitivity, rapid operation, and low reagent consumption, making it a promising candidate for biomedical monitoring applications.
