Antibody-based Electrochemical Biosensors
نویسندگان
1 Department of Biophysics, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, namin, Iran.
2 Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran
3 Department of Biophysics, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, namin, Iran.
doi
10.22036/abcr.2025.529958.2370چکیده
Electrochemical immunosensors are a type of biosensor that combines the principles of electrochemistry and immunology to detect specific biomarkers or analytes in a sample. These sensors typically consist of a transducer, a recognition element, and a signal processing unit. The role of the transducer is to transform the biochemical reaction occurring between the analyte and the recognition component into an electrical output. This can be achieved through various electrochemical techniques, such as amperometry, potentiometry, Voltammetry or conductometry. The recognition element is usually an antibody or an antigen that is immobilized on the surface of the transducer. When the analyte binds to the recognition element, it triggers an alteration in the electrical properties of the transducer, which is then measured as a signal. The signal processing unit is responsible for amplifying and processing the electrical signal to produce a measurable output. This output can be in the form of a current, voltage, or resistance, and can be used to quantify. The level of the analyte present in the sample. Biosensors Based on Electrochemical Immunoassays have been widely used for the detection of various biomarkers, including proteins, hormones, and antibodies. They offer several advantages over traditional methods, including high sensitivity, specificity, and speed.