فهرست نشریات علمی دسترسی آزاد ایران

A highly sensitive and reliable ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for the simultaneous determination of four nitrofuran metabolites (NFMs), namely 3-amino-2-oxazolidinone (AOZ), 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), 1-aminohydantoin (AHD), and semicarbazide (SEM), in aquatic products and seafood. The method involved a simple and efficient sample preparation procedure based on liquid-liquid extraction (LLE), which significantly reduced analysis time and lowered the limit of detection (LOD) to 0.003 µg/kg. The method exhibited excellent linearity (R2 > 0.9998), satisfactory recovery (94-112.9 %), and good repeatability with relative standard deviation (RSD) values below 12%. The validated method was successfully applied to analyze 237 shrimp samples, 12 frog samples, 7 catfish samples, 10 seafood roll samples, and 10 squid samples collected from markets in Ho Chi Minh City, Vietnam. The results revealed the presence of AOZ in 5.91 % of shrimp samples, 14.3 % of catfish samples, and 25 % of frog samples, with concentrations ranging from 0.11 to 22.9 µg/kg. These findings highlight the importance of establishing and enforcing effective control measures to ensure food safety and protect consumer health.