فهرست نشریات علمی دسترسی آزاد ایران

Dengue hemorrhagic fever (DHF) is still a serious health problem in Indonesia. Early detection of the NS1 antigen is an important approach because this antigen appears from the first day of infection. This study aims to develop a colorimetric biosensor based on gold nanoparticles (AuNPs) for the rapid and selective detection of NS1 antigen. AuNPs were synthesized using α-cyclodextrin (α-CDs) as a reducing and stabilizing agent through a heating method at 98°C for 15 minutes. Furthermore, the surface of AuNPs was modified with mercaptoundecanoic acid (MUA) to add carboxylic groups, activated with EDC/NHS to allow bioconjugation with NS1 antibodies (AuNPs-mAb). The UV-Vis characterization results showed a shift in the Surface Plasmon Resonance (SPR) peak from 517 nm to 530 nm after interaction with the NS1 antigen, accompanied by a decrease in absorbance from 0.304 to 0.108 and a color change in the solution from reddish-purple to faint purple in less than 5 minutes. The biosensor showed good sensitivity with a Limit of Detection (LOD) of 22.02 ng mL-1, linearity R² = 0.996, and stability up to 14 days. Selectivity testing against interferents such as glucose, cholesterol, and uric acid showed no cross-reactivity. Applicative tests with human serum proved the effectiveness of detection in real conditions. With the advantages of a fast, practical method that does not require complex equipment, this AuNPs-mAb biosensor has the potential to be an efficient early diagnostic tool for dengue virus infection. It can be further developed for nanoparticle-based clinical applications.