فهرست نشریات علمی دسترسی آزاد ایران

Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used painkillers but can have side effects, making it essential to measure their concentration in biological fluids. In this study, a CoFe2O4@HKUST-1 coating was applied to a capillary glass tube via a hydrothermal method. The coated tube was then used as a solid-phase microextraction (SPME) fiber to detect four NSAIDs (Ibuprofen, Nalidixic acid, Diclofenac, and Naproxen) using HPLC with a UV detector in plasma, urine, and serum samples. Optimization of variables (the initial pH of the sample solution, concentration of salt, time of extraction, and rate of stirring) was done with the Box-Behnken design and one-variable-at-a-time method (pH, type and volume of desorption solvent). The linear range (LR) was 0.06-450 μg L−1 with R2>0.9970, and the limit of detection (LOD) and quantification (LOQ) were 0.01-0.04 μg L−1 and 0.04-0.14 μg L−1, respectively. The relative standard deviation percentage (RSD %) was 1.33-5.43%, and recoveries ranged from 94.45% to 99.00%. This fiber is cost-effective, durable (>112 extractions), and demonstrates superior LOD and LR compared to other methods, making it ideal for monitoring NSAID levels. The magnetic HKUST-1 coating enhances extraction efficiency two to four times compared to nonmagnetic HKUST-1, making CoFe2O4@HKUST-1 a highly efficient sorbent.