Development of an Eu3+ Functionalized Metal-Organic Framework-based Nanofibrous Sensor for Biomedical Monitoring of Hippuric Acid in Urine Samples
نویسندگان
1 Occupational Health and Safety Research Center, Institute of Health Sciences and Technologies, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran Center of Excellence for Occupational Health Engineering,
2 Department of Chemistry, Bu-Ali-Sina University, Hamedan, Iran
3 Occupational Health and Safety Research Center, Institute of Health Sciences and Technologies, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran Center of Excellence for Occupational Health Engineering,
4 Occupational Health and Safety Research Center, Institute of Health Sciences and Technologies, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran Center of Excellence for Occupational Health Engineering,
5 Department of Chemistry, Bu-Ali-Sina University, Hamedan, Iran
6 Occupational Health and Safety Research Center, Institute of Health Sciences and Technologies, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran Center of Excellence for Occupational Health Engineering,
doi
10.22036/abcr.2025.412755.2215چکیده
The current study focuses on the development of a rapid and cost-effective photoluminescence sensor for quantifying hippuric acid (HA) in human urine. This sensor utilizes Eu3+-functionalized metal-organic framework (MOF) embedded in polyacrylonitrile (PAN) nanofibers. The electrospinning technique was employed to enhance the stability and performance of the Eu3+@Cu-BTC embedded PAN nanofibers.The prepared sensor demonstrated acceptable selectivity and sensitivity, exhibiting a linear relationship between the logarithm of HA concentration (Lg [HA]) and the response of both the powder and nanofiber sensors, with R² values of 0.993 and 0.991, respectively. Furthermore, the limit of detection (LOD) was determined to be 3.7 μg/mL for the powder sensor and 1.2 μg/mL for the nanofiber sensor, both significantly below the American Conference of Governmental and Industrial Hygienists (ACGIH) Biological Exposure Index (BEI) for HA, which is set at 2 mg/mL.This research highlights the advantages of electrospinning-based sensors using MOFs, including reusability, reduced measurement errors, user-friendliness, and simplified sample preparation. These features underscore their potential for rapid biological monitoring of toluene exposure in occupational settings.
