A Sensitive Colorimetric Assay for Acetylcholinesterase Activity based on Aggregation of Gold Nanoparticles with Phosalone
نویسندگان
1 Department of Food Science and Technology, Tarbiat Modares University, Tehran, Iran. Professor Massoumi Laboratory, Department of Chemistry, Shiraz University, Shiraz 71454, Iran
2 Department of Food Science and Technology, Faculty of Agriculture, Jahrom University, Jahrom, Iran
doi
10.22036/abcr.2024.426287.2006چکیده
A highly sensitive, fast and convenient colorimetric assay for acetylcholinesterase (AChE) activity was developed. To do this, phosalone was used to aggregate citrate-coated gold nanoparticles which caused its color to be changed from wine-red to blue. This aggregation was significantly hindered with AChE, due to its preferential reaction with phosalone, and the corresponding color change was probed spectrophotometrically as a function of AChE activity. The ratio of the measured absorbances at the wavelengths of 520 and 650 nm (A520/A650) was found to be linear with the activity of AChE from 0.050 to 1.0 U mL−1 (R2 = 0.9912). A limit of 0.030 U mL−1 for detection of AChE was achieved based on signal to noise ratio of 3. The procedure was successfully applied for determination of AChE in real samples as well as quantitation of phosalone in diverse fields such as food and agricultural industries as well as pharmaceutical, healthcare and environmental affairs.
