فهرست نشریات علمی دسترسی آزاد ایران

Cassia tora Linn (Caesalpiniaceae) is a medicinally significant plant that is abundant in anthraquinone derivatives, notably rhein and emodin. The objective of this work was to separate and describe these chemicals using a simple solvent extraction and purification method followed by recrystallization. Structural confirmation was achieved by UV, IR, and mass spectroscopic techniques. Additionally, a validated HPTLC technique was established for the concurrent measurement of rhein and emodin in the seed extracts. The separation by chromatography was improved by applying silica gel plates with a mobile phase of chloroform:methanol (10:0.2 v/v), and the analysis was performed at 254 nm. The concentrations of emodin and rhein have been found to be 0.012% and 0.011%, significantly. Validation in accordance with ICH guidelines confirmed accuracy, precision, specificity, and reproducibility, with mean recovery rates of 95% for emodin and 98% for rhein. The established protocol offers a reliable, cost-effective, systematic, and replicable method for the standard analysis of anthraquinone derivatives in Cassia tora and related species.