فهرست نشریات علمی دسترسی آزاد ایران

This study reports the synthesis of several heterocyclic pharmaceutical compounds, including azetidine-4-one derivatives, obtained via the reaction of preformed hydrazones with dioxane chloroacetyl chloride using a conventional method in the presence of triethylamine as a catalyst. The structural integrity and purity of the synthesized compounds were confirmed through Fourier transform infrared (FTIR) spectroscopy, 1H and 13C-NMR spectroscopy, and elemental analysis (C, H, and N). Reaction progress and completion were monitored using thin-layer chromatography (TLC). The antibacterial activity of the compounds was assessed using the diffusion method at three concentrations (0.01, 0.001, and 0.0001 mg/mL) against two bacterial strains. Ampicillin served as a reference antibiotic. Among the tested compounds, (M7) exhibited the highest inhibitory effect against Staphylococcus aureus, with a zone of inhibition measuring 22 mm. Against Escherichia coli, compounds (M7 and M8) demonstrated the most potent activity, reaching inhibition zones of up to 25 mm. A positive correlation was observed between compound concentration and antibacterial efficacy, indicating that higher concentrations resulted in greater inhibition of bacterial growth.