Chemical Binding Free Energy and Anti-Free Radical of Chemical Compounds from Sea Cucumber (𝑯𝒐𝒍𝒐𝒕𝒉𝒖𝒓𝒊𝒂 𝒔𝒄𝒂𝒃𝒓𝒂) Against Malondialdehyde and Akt
نویسندگان
1 Department of Pharmacology, Faculty of Medicine and Health Science, Universitas Mataram, Mataram, Indonesia
2 Department of Parasitology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
3 Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
4 Department of Radiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
5 Department of Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
doi
10.48309/ajca.2025.518195.1827چکیده
This study investigates the potential protective effects of Holothuria scabra extract against diethylnitrosamine (DEN) and carbon tetrachloride (CCl4)-induced hepatocellular carcinoma in a rat model. Molecular docking predictions revealed (3β,24R,24'R)-fucosterol epoxide as a promising bioactive compound, demonstrating remarkable AKT inhibition with a binding energy of -10.58 kcal/mol. Despite low TFC (1.03 mg QE/g), TPC (0.82 mg GAE/g), and in vitro antioxidant activity (IC50: 2.791 mg/mL), H. scabra extract exhibited potent in vivo therapeutic effects. Experimental validation demonstrated a significant reduction in Akt serum levels by 69.5 nmol/mL and a substantial decrease in malondialdehyde levels by 0.6537 nmol/mL, even lower than the baseline. The extract's capacity to inhibit Akt phosphorylation and alter cellular signalling pathways indicates a new strategy for anti-carcinogenic intervention. These results underscore sea cucumber as a promising natural product with potential therapeutic uses in the management of hepatocellular carcinoma, linking molecular docking predictions with substantial in vivo experimental confirmation.
