The Interplay of p53 Gene Expression and Inflammatory Markers as Predictors of ART Success
نویسندگان
1 Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
2 Research scholar, Meenakshi Academy of Higher Education and Research (MAHER- Deemed to be University), West K.K Nagar, Chennai, Tamil Nadu, India
3 Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
4 Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
5 Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
6 Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
7 Central Research Laboratory, Meenakshi Academy of Higher Education and Research, (Deemed to be University), Chennai, Tamil Nadu, India
8 Registrar, Meenakshi Academy of Higher Education and Research, (Deemed to be University), Chennai, Tamil Nadu, India
9 CRAFT Hospital and Research Centre, Kodungallur Eriyad Road, Opp Seashore Residency, Kodungallur, Kerala, India
10 Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
11 Department of Genetics, Genetika Research Centre for Advanced Genetic Studies, Thiruvananthapuram, Kerala, India
12 Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
13 Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
doi
10.24200/jogcr.11.2.101چکیده
Background & Objective: Infertility affects 10-15% of couples globally, with ART improving success rates. However, implantation failure remains a challenge, influenced by p53 gene expression and inflammatory markers (TNF-α, IL-6). Understanding their interplay could enhance ART outcomes. The present study was conducted with aim to examine how p53 gene expression and inflammatory markers influence endometrial receptivity and implantation.Materials & Methods: This case-control study was conducted on 600 adults from hospitals across Kerala, with 150 infertile couples (cases) and 150 fertile couples (controls) matched by age (34.5±4.9 years). IL-6 and TNF-α levels were measured using enzyme immunoassays, while p53 gene expression was assessed via Real-Time PCR. The associations between inflammatory markers, p53 expression, and implantation failure were evaluated. Data were analyzed using Stata 17.0 and RStudio, with P<0.05 considered significant.Results: Significant differences were observed between cases and controls, with elevated TNF-α, IL-6, and p53 expression in both sexes (P<0.001). ROC analysis showed that IL-6 and p53 had moderate predictive power for implantation failure. Stepwise regression indicated that IL-6 and TNF-α contributed to male infertility, while IL-6 had a strong correlation in females.Conclusion: Elevated IL-6 and TNF-α correlate with increased p53 expression, impacting implantation. While excessive p53 may hinder implantation, controlled inflammation supports receptivity. Targeted interventions, such as anti-inflammatory therapies or p53 modulators, could improve ART outcomes. A deeper understanding of these interactions may lead to personalized treatments for enhanced reproductive success.
