Synthesis and characterization of coumarin-loaded chitosan nanoparticles and their antidiabetic potential in in Vitro
نویسندگان
1 Department of Botany, Bharathiar University, Coimbatore, Tamil nadu-India
2 Department of Botany, Bharathiar University, Coimbatore, Tamil nadu-India
3 Department of Botany, Bharathiar University, Coimbatore, Tamil nadu-India
4 Department of Botany, Bharathiar University, Coimbatore, Tamil nadu-India
5 Department of Botany, Bharathiar University, Coimbatore, Tamil nadu-India
6 Department of Botany, Bharathiar University, Coimbatore, Tamil nadu-India
7 Department of Botany, Bharathiar University, Coimbatore, Tamil nadu-India
doi
10.22036/ncr.2026.482285.1424چکیده
Coumarins are well-known phenolic compounds utilized in medicine for their pharmacological and biological properties. Recent research has concentrated on the development of nanoscale drug-delivery devices that precisely control the release of chemotherapeutic drugs directed at specific cancer cells. This study seeks to encapsulate synthetic coumarin within chitosan nanoparticles (CECSNPs) and to assess the in vitro release kinetics and antidiabetic efficacy of the encapsulated coumarin. CECSNPs were evaluated for entrapment efficiency, drug release percentage, drug release kinetics, UV-Vis spectroscopy, FTIR, XRD and SEM. CECSNPs exhibiting elevated entrapment efficiency underwent multiple in vitro antidiabetic evaluations. Among the different concentrations of CECSNPs, 30 µg/mL exhibited the highest entrapment efficiency percentage (91%). In vitro drug release tests demonstrated a controlled and sustained release of coumarin from micro chitosan (96.66%) over three hours. This study found that the release kinetics of coumarin from CECSNPs was most accurately described by zero-order kinetics, with a correlation coefficient of 0.98. In in vitro anti-diabetic evaluations, CECSNPs at 30µg/mL exhibited the most significant inhibitory effect against α-amylase and α-glycosidase, along with increased glucose absorption.
