فهرست نشریات علمی دسترسی آزاد ایران

In this study, in vitro regenerated Solanum nigrum extract was used for green synthesis of gold nanostructures (Sn-AuNPs), and their anticancer activity was tested using MTT assay against human breast cancer cells (MDA-MB-231). The characterization of Sn-AuNPs was studied using UV–visible spectrophotometry, FESEM, EDX, XRD, DLS, Zeta potential, and FT-IR analyses. TPC and TFC of Sn-AuNPs were evaluated using colorimetric analysis.  DPPH and FRAP tests were applied to confirm their antioxidant potential. Characterization revealed a surface resonance peak at 561 nm, confirming Sn-AuNPs formation. The Sn-AuNPs were monodispersed, spherical, and ranged from 10-26 nm. EDX confirmed the presence of gold, while FT-IR identified flavonoids, tannins, and phenolic compounds as stabilizers. XRD confirmed the crystalline nature, and DLS showed size variation and monodispersity. Zeta potential analysis indicated a negatively charged, stable surface. High phytochemical levels in Sn-AuNPs suggested strong antioxidant properties, confirmed by DPPH assay. Sn-AuNPs showed selective anticancer efficacy against MDA-MB-231 cells with IC50 of 589.17 µg/mL. They had minimal impact on HFF2 cells, even at 500 μg/mL (93.44%), suggesting selective targeting of cancer cells. The anticancer efficacy against MDA-MB-231 cells highlights the Sn-AuNPs' potential for therapeutic applications.