Viral Co-Infection Diagnosis in Cervical Samples Using Next Generation Sequencing Technology
نویسندگان
1 Department of Biology, College of Educations for Pure Science, University of Diyala, Diyala, Iraq
2 Department of Biology, College of Educations for Pure Science, University of Diyala, Diyala, Iraq
3 Department of Microbiology, Collage of Medicine, Diyala University, Diyala, Iraq
doi
10.30699/jogcr.9.5.549چکیده
Background & Objective: The study explores human papillomavirus (HPV), a significant cause of cervical cancer, and its potential coinfections with other viruses like human herpes virus type 4 (HHV-4) or Epstein-Barr virus (EBV) and HIV. Next-generation sequencing (NGS) technology is used to analyze viral content in cervical samples from Iraqi women.Materials & Methods: Eight cervical samples were collected and processed using Illumina DNA Prep on the iSeq100 System. Viral genomic DNA was isolated and libraries prepared for sequencing.Results: MetaviralSPAdes assembled contigs from each sample, which were then blasted against the NT database. HPV16, HHV4, HIV, and bovine alpha herpesvirus 5 were among the viruses identified across samples.Conclusion: Viral coinfections identified through NGS underscore their potential impact on cervical health, emphasizing NGS's diagnostic sensitivity for viral infections not typically detected by standard gynecological methods.
