Eco-friendly Synthesis of CeO2 Nanoparticles Using Bee Pollen: Dye Purification, Antioxidant, Antibacterial, Antifungal and Anticancer Properties
نویسندگان
1 Department of Chemistry, KLE Society's S Nijalingappa College, Rajajinagar, Bengaluru, India
2 Department of Studies in Chemistry, Karnatak University, Pavate Nagar, Dharwad, India
3 MS Ramaiah College of Arts, Science and Commerce, Bengaluru, India
4 Department of Studies in Chemistry, Bangalore University, Bengaluru-560056, Karnataka, India.
5 Department of Studies in Physics, Bangalore University, Bengaluru, India
6 MS Ramaiah College of Arts, Science and Commerce, Bengaluru, India
doi
10.22036/ncr.2025.512371.1460چکیده
Cerium oxide nanoparticles (CeO2 NPs) have an extensive range of uses in the biomedical field due to their outstanding physicochemical and catalytic properties. The present work aims to prepare the CeO2 via a green approach using bee pollen (BP) as a reducing agent. The eco-friendly synthesis of CeO2 with BP was confirmed using UV-Vis, EDX, FTIR, SEM, XRD, and PL techniques. The band gaps of BP, CeO2 and BP/CeO2 were determined to be 4.93, 5.56 and 3.89 eV from absorption measurements using Tauc plots. The room temperature photoluminescence results for BP and BP/CeO2 revealed emission bands at 310, 340 and 380 nm and the UV absorption spectrum was estimated at 269, 272 and 309 nm, respectively. The photocatalytic efficiency of BP/CeO2 was enhanced up to 86 % for BP/CeO2 when compared to that of BP and CeO2 against Rose Bengal (RB) dye. The antioxidant activity was enhanced (97.24%) with a decreased IC50 value of around 154.64 mg/µL against DPPH radical. The antibacterial and antifungal properties of BP/CeO2 were investigated against gram-positive and gram-negative bacteria (Bacillus subtilis and Escherichia coli). These findings indicate a controlled impact of zone inhibitors in increasing concentrations. Penicillium sp. exhibited higher inhibitory zones in both samples than Aspergillus niger. The produced samples have been evaluated for anticancer activity utilizing doxorubicin on HeLa cells, which showed better results.
