Green Synthesis and Characterization of Selenium Nanoparticles from Khaya grandifoliola, and Assessment of their Antioxidant, Antidiabetic, Anticholinesterase, and Anticancer Potential
نویسندگان
1 Department of Biotechnology and Food Science, Durban University of Technology, Durban, South Africa.
2 Department of Chemistry, Federal University Oye-Ekiti, Ekiti state, Nigeria.
3 Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria.
4 Department of Chemistry, Federal University Oye-Ekiti, Ekiti state, Nigeria.
5 Nanobiochemistry Research Group, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria.
6 Biomembrane, Phytomedicine, and Drug Development Unit, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria.
7 Centre of Excellence for pharmaceutical sciences, Northwest University, Potchefstroom, South Africa.
8 Plants for Biotechnological Resources Research Group, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria.
doi
10.22036/ncr.2025.480925.1420چکیده
Selenium nanoparticles (SeNPs) are emerging as a promising area of nanotechnology due to their diverse biomedical potential. Green synthesis offers significant advantages by addressing the need for environmentally friendly, non-toxic, and cost-effective methods. This study investigates the green synthesis of SeNPs using the ethanolic root bark extract of Khaya grandifoliola (RKG) and evaluates their biological activities, including antioxidant, antidiabetic, anticholinesterase, and anticancer properties. The SeNPs were synthesized using sodium selenite and RKG as a reducing agent, followed by characterization via Fourier Transform Infrared (FT-IR) spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Energy-Dispersive X-ray Spectroscopy (EDX). A color change from light yellow to ruby-red and a UV-visible surface resonance peak at 250 nm confirmed SeNP formation. FT-IR confirmed the role of RKG phytochemicals in the synthesis, while SEM and TEM revealed spherical nanoparticles with an average size of 29.34 nm. EDX analysis confirmed the presence of elemental selenium. The RKG-mediated SeNPs (RKGSeNPs) exhibited strong antioxidant activity, with IC50 values of 50, 40, and 35 µg/ml for DPPH, nitric oxide, and ferric ion radicals, respectively. They also inhibited α-amylase (IC50 48 µg/ml) and α-glucosidase (IC50 55 µg/ml), demonstrating potential antidiabetic properties. Additionally, RKGSeNPs inhibited acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with IC50 values of 25 µg/ml and 22 µg/ml, respectively. Notably, they also exhibited antiproliferative effects on breast cancer cells (MCF-7) with IC50 of 35 µg/ml. These findings suggest that RKGSeNPs can be further explored for antioxidant, antidiabetic, anticholinesterase, and anticancer applications.
