Extraction, Phytochemical Screening, Green Synthesis of Silver Nanoparticles (Ag-NPs), and Antioxidant Potential of Aphanamixis polystachya
نویسندگان
1 Department of Pharmacology, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai - 600 117, India
2 Department of Pharmaceutical Chemistry and Analysis, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai - 600 117, India
3 Department of Pharmaceutics, School of Pharmaceutical sciences, VELS Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai - 600 117, India
4 Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraidah 51452, Saudi Arabia
5 Department of Psychiatry, SRM Medical College Hospital and Research Centre, SRMIST, SRM Nagar, Kattankulathur - 603 203. Chengalpattu District, Tamil Nadu India
6 Department of Pharmaceutics, School of Pharmaceutical Sciences, VELS Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai - 600 117, India
7 Department of Pharmaceutical Chemistry, Government College of Pharmacy, Osmanpura, Chhatrapati Sambhajinagar, Pin 431005, Maharashtra, India
8 Department of Pharmaceutical Chemistry & Analysis, School of Pharmaceutical Sciences, Vels Institute of Science, Technology & Advanced Studies, Pallavaram, Chennai - 600 117, Tamil Nadu, India
doi
10.48309/jaoc.2025.525464.1299چکیده
This study reports the green synthesis of silver nanoparticles (Ag-NPs) using an aqueous leaf extract of Aphanamixis polystachya, a plant known for its medicinal properties. The biosynthesized Ag-NPs were characterized using UV-Vis spectroscopy, FTIR, SEM, XRD, DLS, and zeta potential analysis. The UV-Vis spectrum displayed a surface plasmon resonance peak at 425 nm, and particle size analysis showed an optimized average diameter of 39.7 nm. Zeta potential was recorded at -9.29 mV, confirming colloidal stability, while entrapment efficiency reached 82.2%. Functional groups such as O–H, C=O, and C–N identified by FTIR analysis facilitated nanoparticle formation and stabilization. SEM revealed spherical morphology and XRD confirmed a crystalline face-centered cubic structure with peaks at 38.1°, 44.3°, 64.4°, and 77.4°. In vitro antioxidant evaluation using DPPH assay demonstrated superior activity of Ag-NPs (IC50 = 0.68 ± 0.06 mg/mL) over the crude leaf extract (IC50 = 1.57 ± 0.07 mg/mL). Additionally, the Ag-NPs exhibited a drug release of 98.27 ± 2.76% over 12 hours and remained physically stable over three months. These findings imply that the leaf extract of A. polystachya functions as a sustainable, eco-friendly, and efficient reducing and capping agent for the production of stable Ag-NPs, with encouraging prospects for additional biological and medicinal uses.
