Synthesis and Characterization of Acetamidotetrazole-Grafted Magnetic Chitosan Biopolymer as a Novel Non-Virus Vector for Targeted Gene Delivery into HECK-293T Cells
نویسندگان
1 Department of New Materials, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. IRAN
2 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. IRAN
3 Department of New Materials, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. IRAN
doi
10.30492/ijcce.2024.1973567.5719چکیده
This study successfully synthesized a magnetic chitosan biopolymer covalently grafted onto acetamido tetrazole and evaluated it as a high-potential biocompatible non-viruses vector. The synthesized nanocarrier was fully characterized by FT-IR spectra, TEM and FESEM images, XRD, EDX, FESEM, and DLS analyses. The loading capacity of the plasmid was optimized with the ratio N/P 3 with excellent protection of the plasmid. The nanocarrier showed high gene expression and transfection in the HECK-293T cell line without any inherent toxicity. In addition, experimental results show that the new functionalized chitosan has excellent DNA condensation, protection, and high target transfection efficiency. Therefore, the novel magnetic chitosan nanocarrier functionalized with acetamido tetrazole has shown potential uses in cancer therapy.
