Biophysical Effect of Anionic Surface-Active Substance on the Growth Dynamic and Computational Predict in Extracellular Signal-Regulated Kinases (ERKs) of Dunaliella viridis Microalgae
نویسندگان
1 Assistant Professor of Molecular Biophysics, Faculty of Biological Science, Department of Molecular and Cell Biochemistry, Falavarjan Branch , Islamic Azad University, Isfahan, Iran
2 Assistant Professor of Biology, Faculty of Natural Resources and Marine Science, Department of Marine Biology, Tarbiat Modares University, Iran
doi
10.22108/ijpb.2018.105517.1039چکیده
The present study was conducted to evaluate the anionic SDS surfactant in various environmental conditions on Dunaliella viridis alga growth dynamics. The effect of different concentrations of 0.1, 1 and 10 mg. L-1 of SDS and pH of 7, 8, 9 and 10 on the Dunaliella were investigated. The 20% reduction in growth rate of algae in all concentrations in comparison with the control was observed in the sixth and seventh day of experiments. The growth rate was close to 92% in the culture medium at a concentration of 1 mg. L-1 SDS at pH 8 on day 7. In addition, the biodegradation process of surfactant will depend on the length of the alkylated chain as well as to cell metabolism. The short-lived alkyl chain SDS showed the highest percentage of degradation in the cell population. Therefore, the loss of cell density caused by surfactant in the culture medium results in an effect on the Dunaliella cell cycle, which is strongly dependent on ERK phosphorylation of MAPKs. In this research, to predict the structure and function of protein, the models have used the multiple sequencing alignment algorithms more. ERK sequences showed that their residues are strongly conserved in unicellular microalgae in higher plants and mammals. The degree of identity between the model sequences (Target) and ERK1 and ERK2 protein kinases (Template) has been calculated in RMSD 2.30 and 2.9 Å with highest sequence coverage.
