فهرست نشریات علمی دسترسی آزاد ایران

A high-performance thin-layer chromatography (HPTLC) method was developed and validated to quantify furosemide in human plasma, providing a rapid, cost-effective, and environmentally sustainable alternative to conventional chromatographic techniques. The method employed silica gel 60 F254 precoated TLC plates with a mobile phase consisting of isopropyl alcohol, formic acid, ethyl acetate, chloroform, and toluene (1.5:1:0.5:3:4, v/v/v/v/v), and densitometric detection at 271 nm. Paracetamol was used as an internal standard. The method exhibited a strong linear correlation over the 300-2100 ng/band concentration range with a correlation coefficient (r²) of 0.9949. The lower limit of quantification was 50 ng/band, demonstrating high sensitivity. The intra-day and inter-day precision (%CV) were less than 2%, and the accuracy ranged from 85.49% to 94.46%. Recovery was consistent across all QC levels, with a maximum of 84.70%, and dilution integrity yielded 111% recovery. The method proved robust under slight variations in chromatographic conditions and showed acceptable stability under benchtop, freeze-thaw, overnight, and long-term storage conditions. Overall, this HPTLC method is simple, reproducible, and suitable for high-throughput applications, such as pharmacokinetic studies, bioequivalence assessments, and therapeutic drug monitoring of furosemide in human plasma.