A Sensitive Analytical Approach for the Spectrophotometric Detection of Sulfamerazine in Its Medicinal Formulations Utilizing N, N-Dimethyl-P-Phenylenediamine Dihydrochloride as a Coupling Agent
نویسندگان
1 Department of Chemical Engineering, College of Engineering, Tikrit University, Tikrit, IRAQ
2 Department of Chemistry, College of Education for Women, Tikrit University, Tikrit, IRAQ
doi
10.30492/ijcce.2025.2066996.7213چکیده
One aim of the study was to create a spectroscopic method for evaluating the drug sulfamerazine. The current method creates a violet-colored soluble product by employing an oxidative coupling reaction between sulfamerazine (SMZ) solution and N, N-dimethyl-p-phenylenediamine dihydrochloride solution (N, N-DMPPDADH) in basic medium with sodium periodate (NaIO4) as an oxidizing agent. A colored product demonstrates maximum absorption at a wavelength of 564 nm. The concentration range of 3.125 - 50 µg/mL of sulfamerazine is covered by the technique under evaluation, based on Beer's law. The sensitivity of the technique is shown by the specific molar absorbance value of 9346.77 L/(mol.cm) and the Sandell significant value, or sensitivity per unit length of 0.0271 μg/cm2. The study is shown to have exceptional precision, which is corroborated by the low relative standard deviation value of 0.79%. This method's validated detection limit of 0.0124 μg/mL demonstrates how accurate it is in recognizing sulfamerazine at such low amounts. The method that was created has been beneficial in sulfamerazine screening for medicinal items. The reported 99.98% recovery rate of sulfamerazine in the studied samples indicates that the approach was successful in precisely determining the sulfamerazine content of real-world samples.
