Toll-Like Receptors Expression in Follicular Cells of Patients with Poor Ovarian Response
نویسندگان
1 Department of Obstetrics and Gynecology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, R
2 5Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
3 Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
4 Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;4Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
5 Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;4Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
doi
چکیده
Background Poor ovarian response (POR) to gonadotropin stimulation has led to a significant decline in success rate of fertility treatment. The immune system may play an important role in pathophysiology of POR by dysfunctions of cytokines and the growth factor network, and the presence of ovarian auto-antibodies. The aim of this study is to investigate the expression of toll-like receptors (TLR) 1, 2, 4, 5, 6 and cyclooxygenase (COX) 2 genes in follicular cells and concentration of interleukin (IL)-6, IL-8 and macrophage migration inhibitory factor (MIF), as major parts of innate immunity, in follicular fluid (FF) obtained from POR women in comparison with normal women. Materials and Methods In this case-control study, 20 infertile POR patients and 20 normal women took part in this study and underwent controlled ovarian stimulation. The FF was obtained from the largest follicle (>18 mm). The FF was centrifuged and cellular pellet was then used for evaluation of expression of TLRs and COX2 genes by real-time PCR. FF was used for quantitative analysis for IL-6, IL-8 and MIF by enzyme-linked immunosorbent assay (ELISA). Results TLR1, 2, 4, 5, 6 and COX2 gene expression were significantly higher in POR (p < 0.05). Concentration of IL-6, IL-8 and MIF proteins was significantly increased in POR compared with normal women (p < 0.05). Conclusion These findings support the hypothesis that the immune system may be involved in pathophysiology of POR through TLRs.
