Application of the Taguchi Design for Production of Poly(β-hydroxybutyrate) by Ralstonia eutropha
نویسندگان
1 Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University P.O. Box 14115-143, Tehran, I.R. IRAN
2 Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University P.O. Box 14115-143, Tehran, I.R. IRAN
3 Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University P.O. Box 14115-143, Tehran, I.R. IRAN
doi
10.30492/ijcce.2004.8171چکیده
The Taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(PHB) production by Ralstonia eutropha. The optimum condition was obtained as: fructose concentration, 15 g/L; C/N ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° C; seed age, 15 h. At optimum condition the yield of PHB production was found to be 92.36%.
