Behenic Acid Monolayer and Bilayer Assemblies- A Study of Concanavalin A (Con A) Adsorption and its Interaction with Dextran Using Surface Plasmon Resonance Spectroscopy and Microscopy
نویسندگان
1 Chemical Sensor Group, Department of Chemistry, Erindale College, University of Toronto, Mississauga Road, Mississauga, Ontario, L5L 1C6, CANADA
2 Department of Chemistry, Faculty of Sciences, Ferdowsi University Mashhad, I.R. IRAN
3 Chemical Sensor Group, Department of Chemistry, Erindale College, University of Toronto, Mississauga Road, Mississauga, Ontario, L5L 1C6, CANADA
doi
10.30492/ijcce.1993.10930چکیده
Deposition of behenic acid (B.A) mono and bilayers onto gold coated surfaces was performed by Langmuir-Blodget dip-casting technique. Surface Plasmon resonance (SPR) and ellipsometry methods were employed for investigation of the monolayer and bilayer films. The adsorption of the biologically important molecule Concanavalin A (Con A) from bulk solution to these monolayers and bilayers as well as the interaction between deposited Con A with dextran molecules in the presence of Ca2+ and Mn2+ ions was investigated. The results obtained, show that no significant interaction occurs between the adsorbed Con A and dextran in the case of B.A. bilayer, while a significant binding takes place in the monolayer.
