Development of An Immobilized Enzyme Electrode for the Determination of Sulfite Ion
نویسندگان
1 Department of Chemistry and Physical Sciences, Pace University, Pleasantville, New York 10570, USA
doi
10.30492/ijcce.1992.10401چکیده
An enzyme electrode was developed using the enzyme sulfite oxidase (EC1.8.3.1) immobilized onto pig intestine. Hydrogen peroxide produced was monitored amperometrically. All experimental parameters such as pH, temperature, buffer constituent concentration, were thoroughly investigated and optimized when appropriate. The response to sulfite concentration was linear in the range 5.2´10-6 to 1.02´10-3 M. The proposed method was found to have a correlation coefficient of 0.952 when evaluated in comparison with the standard titration method.
