Electrochemical Immunosensor Based on Catalytic Activity of Nitrogen-doped Graphene Quantum Dots Supported by Graphene Nanoribbon Composite for Carbohydrate Antigen 15-3 Detection
نویسندگان
1 Medical Biology Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Pharmaceutical Sciences Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Pharmaceutical Sciences Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
doi
10.22036/abcr.2021.279163.1619چکیده
Due to the inherent properties of electrochemical immunosensors, they are widely considered to detect various biomarkers. This study developed an electrochemical immunosensor via the excellent catalytic effect of nitrogen-doped graphene quantum dot supported by graphene nanoribbon (N-GQD/GNR) composite as a sensing platform. Nafion was used as a binder for binding of N-GQD/GNRon the electrode surface . The modified electrode with N-GQD/GNR composite was applied to develop the label-free electrochemical immunosensor for the measurements of carbohydrate antigen 15-3 (CA15-3) biomarker. The catalytic activity of N-GQD/GNR nanocomposite increases the peak currents of the proposed immunosensor due to the accelerated electron transfer between the sensing platform and probe. This immunosensor has a wide linear range of 0.5 to 150.0 U mL-1, with a detection limit of 0.1 U mL-1. Also, the proposed immunosensor has significant specificity, high sensitivity, and accuracy. The prepared electrochemical immunosensor was used to detect CA15-3 protein in blood samples.
