Solid Phase Extraction of Letrozole by a Metal Organic Framework and its HPLC-UV Characterization
نویسندگان
1 Nano Drug Delivery Research Center, Health Technollogy Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Department of Chemistry, Tarbiat Modares University, P. O. Box: 14115-175, Tehran, Iran
3 Nano Drug Delivery Research Center, Health Technollogy Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
doi
10.22036/abcr.2021.245300.1534چکیده
We successfully synthesized TMU-24 as metal-organic framework (MOF) and employed as an effective sorbent for preconcentration of the letrozole (LTZ) from biological samples. Using high-performance liquid chromatography with UV detector (HPLC-UV) coupling with solid-phase extraction (SPE) a sensitive, fast, and simple method for deteing of LTZ has been developed. The optimal conditions, such as pH, type of eluent solvents, contact time amount of adsorbent, and adsorption capacity were discussed. Under the optimized conditions, relative recoveries (RR) and preconcentration factor (PF) of the LTZ was obtained in the range of 92.4–99.6% and 33 respectively. Under the optimal conditions, LTZ can be determined with a limits of detection (LODs) 0.5 μg L-1 (based on S/N = 3), a linearity in the range of 1–250 μg L-1, and a reasonable linearity of 0.995. Also, the experimental adsorption isotherms models were investigated. The developed method was successfully used to the analysis of LTZ in urine samples.
