فهرست نشریات علمی دسترسی آزاد ایران

Introduction: Important genes that may be expressed in women candidates for mastectomy due to breast cancer are important. The expression of these genes in these women can play an important role in the treatment of these patients. The aim of this study was to investigate the expression level of glutathione peroxidase and glutathione reductase genes in mastectomies women. Procedure: After cell culture, the cell suspension was planted in 96-well microplates and treated for 24 hours in a CO2 incubator at 37 °C. To synthesize cDNA, extracted RNA molecules were used with the help of Fermentaz Revert Aid TM kit, produced in the United States. Results: The concentration of 500 micrograms of nanoparticles caused the death of more than 60% of MCF 7 cells (P< 0.001) while there was no significant difference in all concentrations of nanoparticles on the normal HEK293 cell line. Conclusion: The anti-tumor effects of zinc oxide nanoparticles were shown in increasing the expression of glutathione peroxidase and glutathione reductase genes in MCF-7 breast cancer cell line of mastectomy candidates.