Studies on the Binding of DNA with the Inclusion of Brilliant Green inside the Cavity of γ-Cyclodextrin
نویسندگان
1 Department of Chemistry, College of Material Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010, CHINA
2 Department of Chemistry, College of Material Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010, CHINA
doi
10.30492/ijcce.2013.5866چکیده
The interaction of brilliant green with herring sperm DNA was investigated in detail by spectrometric methods in γ-cyclodextrin systems. On the condition of physiological pH, brilliant green prefers to form the 1:1 inclusion complex with γ-cyclodextrin.All the evidences indicated that the binding modes between γ-cyclodextrin-brilliant green and DNA were grooving binding and partial non-classical intercalative binding. The binding ratio of the inclusion complex with DNA is 6:1. The calculated thermodynamic parameters suggested that the binding of the inclusion complex to DNA was driven mainly by entropy.
